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KMID : 1189420160290040024
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2016 Volume.29 No. 4 p.24 ~ p.33
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Anti-Inflammatory Effects of Tongbi-san(通痺散) Extract on RAW264.7 Macrophages
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Kim Yong-Min
Kim Hee-Taek
Kim Ee-Hwa
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Abstract
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Objectives : This study is to investigate the anti-inflammatory and anti-oxidant effects of Tongbi-san extract (TS) on RAW264.7 macrophages using by cell cytotoxicity, Nitric Oxide (NO) and Prostaglandin E2 (PGE2) production and 1,1-diphenyl-2-picryl ghdrazyl (DPPH) free radical scavenging capability.
Methods : Cell cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The production of NO was measured by Griess assay. The production of PGE2 was measured by immunoassay. And the anti-oxidant activity was measured by the DPPH method.
Results : TS did not increased significantly compared to the TS untreated group in the cell cytotoxicity. TS inhibited NO and PGE2 production in lipopolysaccharide-stimulated RAW 264.7 cells. TS had the DPPH free radical scavenging capability.
Conclusion : The anti-inflammtory and anti-oxidant effects of TS may be use for a treatment of anti-inflammatory diseases.
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KEYWORD
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Anti-inflammation , Anti-oxidant activity , Nitric Oxide , Prostaglandin E2 reactive oxygen species , Tongbi-san
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